MOS-DAD

The MOS-DAD (Multi-wavelength Optical System – Diode Array Detector) from Bio-Logic is the spectral detection module for the SFM stopped-flow platform, recording complete absorption spectra at each time point rather than single-wavelength traces, enabling 3D kinetic spectral analysis.

Spectral acquisition:
– Wavelength range: 200-800 nm (full UV/Vis)
– Detector: 256-element or 1024-element silicon diode array
– Spectral resolution: ~1 nm per pixel
– Acquisition rate: up to 1000 spectra/second
– Each spectrum: complete 200-800 nm scan in <1 ms Advantages over single-wavelength detection: - No need to select monitoring wavelength in advance - Detect multiple intermediates simultaneously at their optimal wavelengths - Global spectral analysis: decompose into pure intermediate spectra - Identify unexpected intermediates not visible at a single wavelength - Validate stoichiometry of chromophore changes Data analysis: - 3D dataset: time x wavelength x absorbance - Global analysis software: SVD decomposition, target analysis - Extract species-associated difference spectra (SADS) - Kinetic traces extractable at any wavelength post-acquisition - BioKine32 integration for acquisition; compatible with third-party global analysis Applications: - Heme protein reactions: track Soret and Q-band changes simultaneously - Flavin/cofactor enzymes: monitor all chromophores at once - Protein conformational changes: UV/Vis spectral shifts - Photochemical reactions: track all photoproducts - Multicomponent reaction systems: resolve overlapping spectra NewRoad provides Bio-Logic MOS-DAD in Israel for multi-wavelength stopped-flow kinetics and spectral evolution studies.