Cryogenic Stopped Flow

The Cryogenic Stopped Flow from Bio-Logic is a temperature-control accessory for Bio-Logic SFM stopped-flow systems, extending the operating range to sub-zero temperatures for cryoenzymology, cold denaturation studies, and trapping of short-lived intermediates.

Temperature range:
– Operating range: -40 to +60°C
– Standard SFM Peltier range: -10 to +70°C
– Cryogenic extension: achieves -40°C with co-solvent buffer
– Coolant: recirculating cryostat required (not included)
– Temperature stability: +/-0.5°C at sub-zero temperatures

Co-solvent requirements:
– Requires cryoprotectant in buffer: glycerol (30-60%), ethylene glycol (30-50%), or DMSO (20-30%)
– Slows reaction rates: allows observation of intermediates normally too short-lived at room temperature
– Maintains solvent viscosity: accounts for viscosity effects in data interpretation

Applications:
– Cryoenzymology: trap and characterize enzyme-substrate intermediates
– Cold denaturation: study protein unfolding at sub-zero temperatures
– Metalloprotein redox: slow electron transfer in Fe/Cu proteins at low T
– RNA folding: trap kinetic folding intermediates
– Protein assembly: study oligomerization intermediates

Compatibility:
– Mounts on SFM-2000, SFM-3000, SFM-4000 mixers
– MOS detection modules: MOS-200, MOS-500, MOS-DAD
– Low-temperature optical cell: compatible with UV/Vis and CD detection

NewRoad provides Bio-Logic Cryogenic Stopped Flow accessory in Israel for research requiring sub-zero temperature kinetics.