SFM-2000

The SFM-2000 from Bio-Logic is the entry-level stopped-flow mixing system in the SFM series, offering two-syringe mixing with sub-2 ms dead time and full compatibility with Bio-Logic MOS optical detection modules.

Mixing performance:
– Dead time: <2 ms standard; <1.5 ms with optimized cell - Drive: pneumatic, computer-controlled - Volume per shot: 80-200 µL (adjustable) - Minimum sample: 0.5 mL per syringe - Throughput: >1 shot per second for kinetics series

Modular design:
– Mounts directly onto MOS-200 or MOS-500 optical module
– Detection options: UV/Vis absorbance, fluorescence, CD (via MOS-200/MOS-500)
– Diode array option: add MOS-DAD for full spectrum per time point
– Upgradeable: SFM-2000 can be upgraded to SFM-3000 with 3rd syringe

Temperature control:
– Syringe block: -10 to +70°C Peltier
– Observation cell: matched temperature control
– Stability: +/-0.1°C
– Important for activation energy and temperature-jump studies

Common applications:
– Enzyme kinetics: Michaelis-Menten pre-steady-state burst
– Protein-DNA binding: association kinetics at various salt
– Folding studies: simple two-state folding kinetics
– Ion channel ligand binding: receptor pharmacology
– Indicator dye kinetics: pH jump, Ca2+ release

Software:
– BioKine32: kinetics acquisition and fitting
– Multi-exponential fitting: 1 to 4 exponentials
– Global analysis across multiple conditions

NewRoad provides Bio-Logic SFM-2000 in Israel as an entry-level stopped-flow system for rapid kinetics research.