SFM-3000

The SFM-3000 from Bio-Logic is a 3-syringe stopped-flow system that adds double-mixing capability over the 2-syringe SFM-2000, allowing sequential mixing experiments to study transient intermediates in multi-step biochemical reactions.

Double-mixing with 3 syringes:
– Syringe 1 + Syringe 2: first mixing event creates intermediate
– Variable aging delay: hold intermediate for defined time (1 ms to seconds)
– Intermediate + Syringe 3: second mixing event probes intermediate reactivity
– Classic application: denature protein, dilute to initiate folding, then quench at defined folding time

Performance:
– Dead time: 1.0 ms (standard cell); <0.8 ms (high-performance cell) - Volume per shot: 80-150 µL per mixing event - Drive: all 3 syringes independently pneumatic with computer-controlled timing - Compatible with MOS-200, MOS-500, MOS-DAD detector modules Detection (via MOS modules): - UV/Vis absorbance: single wavelength PMT - Circular dichroism: CD-detected protein folding - Fluorescence: intrinsic Trp or extrinsic label - Diode array: full spectrum at each time point Key applications: - Protein folding intermediates: kinetic trapping and characterization - RNA folding pathways: double-jump with structure probes - Enzyme pre-steady-state: substrate + cofactor sequential addition - Competitive inhibitor kinetics: pre-formed complex challenged with substrate Temperature control: - Syringe block and cell: -10 to +70°C Peltier - Stability: +/-0.1°C NewRoad provides Bio-Logic SFM-3000 in Israel for research requiring double-mixing stopped-flow capabilities.