SFM-4000

The SFM-4000 from Bio-Logic is the most versatile stopped-flow system in the Bio-Logic SFM series, featuring four independently driven syringes for complex mixing protocols including triple mixing, sequential mixing, and double-jump experiments.

Mixing configurations with 4 syringes:
– 2-syringe mode: standard stopped-flow (2 reagents)
– 3-syringe mode: double mixing, two reagents + one diluent
– 4-syringe mode: triple mixing or sequential double-jump
– Sequential mixing: variable delay between first and second mix
– Allows study of transient intermediates formed between two mixing events

Performance:
– Dead time: 0.8 ms (standard cell); <0.5 ms (optimized) - Drive: all 4 syringes independently pneumatic - Volume per shot: adjustable; 60-200 µL per mixing event - Compatible with MOS-200, MOS-500, MOS-DAD for multi-mode detection Detection options (via MOS modules): - UV/Vis absorbance - Circular dichroism - Fluorescence intensity and anisotropy - Diode array (full spectrum per time point) Applications: - Protein folding intermediates: initiate folding, then probe at defined delay - Enzyme mechanism: substrate + inhibitor sequential addition - RNA assembly: multi-step folding pathway - Receptor pharmacology: agonist pre-incubation before antagonist challenge Instrument design: - Fully automated syringe drive: computer-controlled timing - Temperature control: -10 to +70°C Peltier - Modular: mounts onto MOS-200, MOS-500, or MOS-DAD detector NewRoad provides Bio-Logic SFM-4000 in Israel for research groups studying complex multi-step kinetics.