Rapid Kinetics

Rapid kinetics systems mix reagents in milliseconds and follow the resulting reaction in real time, making it possible to resolve transient intermediates that are invisible to conventional benchtop spectroscopy. NewRoad supplies Bio-Logic stopped-flow, freeze-quench and cryogenic stopped-flow platforms together with the absorbance, fluorescence and circular dichroism detection modules each experiment requires.

How it works

FROM PRINCIPLE TO APPLICATION

Two or more solutions are driven from precision syringes into a micro-volume mixer. Flow is stopped abruptly and the reaction is followed optically from the moment of mixing, with dead times down to roughly one millisecond. Quench-flow and freeze-quench variants instead arrest the reaction chemically or thermally at defined time points for later analysis.

Technologies

Rapid Kinetics

How it works

FROM PRINCIPLE TO APPLICATION

What It Is Used For

Enzyme kinetics and reaction mechanism studies

Protein folding and conformational change

Ligand binding and dissociation rates

Electron transfer in metalloproteins

Fast inorganic and organic reaction mechanisms

Freeze-quench sample preparation for EPR

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